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gen iii microarray spotter  (Amersham Pharmacia Biotech Ltd)

 
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    Amersham Pharmacia Biotech Ltd gen iii microarray spotter
    Gen Iii Microarray Spotter, supplied by Amersham Pharmacia Biotech Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gen+iii+microarray+spotter/gen+iii+microarray+spotter/pm23229724-32-28-32
    Average 90 stars, based on 1 article reviews
    gen iii microarray spotter - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Inverted Microscopy:

    Article Title: Effect of serum from sub-healthy subjects with fatigue on the gene expression profile of skeletal muscle cells.
    Article Snippet: .. A Leica inverted microscope (Wetzlar, Germany), Sigma 3K30 centrifuge (Sigma, St. Louis, MO, USA), incubator (Sun Co., Ltd., Japan), clean bench (Sunshine Experimental Instrument Co., Ltd., Shanghai, China), Gen III Microarray Spotter (Amersham Pharmacia Biotech Ltd., Foster City, CA, USA), Gen III Microarray Scanner (Amersham Pharmacia Biotech, Ltd.), Shel Lab general purpose incubator (Shel Lab, Ltd.), Bio-Rad Mini-Sub GT System (Bio-Rad Ltd., Hercules, CA, USA), DU 520 UV/Vis spectrophotometer (Beckman Coulter Ltd., Miami, FL, USA), Vilber Gel documentation system (Vilber, Ltd.), UV Crosslinker (Vilber Lourmat, France) and MIDAS (Chipscreen Biosciences, Ltd.) were used in the present study. .. A Qiagen RNeasy mini kit (Qiagen, Inc., Hilden, Germany), Qiagen PCR Purification kit (Qiagen, Inc.), CyScribe cDNA Labeling kit (Amersham Pharmacia Biotech, Ltd.), TRIzol reagent (Invitrogen, Carlsbad, CA, USA), DEPC (Amersham Pharmacia Biotech, Ltd.), DMEM/F12, fetal bovine serum (FBS; HyClone, Logan, UT, USA), 0.25% trypsin, serum from sub-healthy subjects with fatigue and normal serum (our lab) were also used in the present study.

    Microarray:

    Article Title: Effect of serum from sub-healthy subjects with fatigue on the gene expression profile of skeletal muscle cells.
    Article Snippet: .. A Leica inverted microscope (Wetzlar, Germany), Sigma 3K30 centrifuge (Sigma, St. Louis, MO, USA), incubator (Sun Co., Ltd., Japan), clean bench (Sunshine Experimental Instrument Co., Ltd., Shanghai, China), Gen III Microarray Spotter (Amersham Pharmacia Biotech Ltd., Foster City, CA, USA), Gen III Microarray Scanner (Amersham Pharmacia Biotech, Ltd.), Shel Lab general purpose incubator (Shel Lab, Ltd.), Bio-Rad Mini-Sub GT System (Bio-Rad Ltd., Hercules, CA, USA), DU 520 UV/Vis spectrophotometer (Beckman Coulter Ltd., Miami, FL, USA), Vilber Gel documentation system (Vilber, Ltd.), UV Crosslinker (Vilber Lourmat, France) and MIDAS (Chipscreen Biosciences, Ltd.) were used in the present study. .. A Qiagen RNeasy mini kit (Qiagen, Inc., Hilden, Germany), Qiagen PCR Purification kit (Qiagen, Inc.), CyScribe cDNA Labeling kit (Amersham Pharmacia Biotech, Ltd.), TRIzol reagent (Invitrogen, Carlsbad, CA, USA), DEPC (Amersham Pharmacia Biotech, Ltd.), DMEM/F12, fetal bovine serum (FBS; HyClone, Logan, UT, USA), 0.25% trypsin, serum from sub-healthy subjects with fatigue and normal serum (our lab) were also used in the present study.

    Spectrophotometry:

    Article Title: Effect of serum from sub-healthy subjects with fatigue on the gene expression profile of skeletal muscle cells.
    Article Snippet: .. A Leica inverted microscope (Wetzlar, Germany), Sigma 3K30 centrifuge (Sigma, St. Louis, MO, USA), incubator (Sun Co., Ltd., Japan), clean bench (Sunshine Experimental Instrument Co., Ltd., Shanghai, China), Gen III Microarray Spotter (Amersham Pharmacia Biotech Ltd., Foster City, CA, USA), Gen III Microarray Scanner (Amersham Pharmacia Biotech, Ltd.), Shel Lab general purpose incubator (Shel Lab, Ltd.), Bio-Rad Mini-Sub GT System (Bio-Rad Ltd., Hercules, CA, USA), DU 520 UV/Vis spectrophotometer (Beckman Coulter Ltd., Miami, FL, USA), Vilber Gel documentation system (Vilber, Ltd.), UV Crosslinker (Vilber Lourmat, France) and MIDAS (Chipscreen Biosciences, Ltd.) were used in the present study. .. A Qiagen RNeasy mini kit (Qiagen, Inc., Hilden, Germany), Qiagen PCR Purification kit (Qiagen, Inc.), CyScribe cDNA Labeling kit (Amersham Pharmacia Biotech, Ltd.), TRIzol reagent (Invitrogen, Carlsbad, CA, USA), DEPC (Amersham Pharmacia Biotech, Ltd.), DMEM/F12, fetal bovine serum (FBS; HyClone, Logan, UT, USA), 0.25% trypsin, serum from sub-healthy subjects with fatigue and normal serum (our lab) were also used in the present study.



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    Fig. 4. Relationship between regulated target genes and the relative drug peroxisome proliferator-acti- vated receptor- (PPAR-) efficacy. A: Comparison of quantitative expression levels as measured in microar- rays and Northern blot analysis and the relative PPAR- efficacies of the tested compounds using linear regres- sion analysis. Fold regulations as calculated in Table 2 were used for the <t>microarray</t> data, whereas signal in- tensities for the genes in Northern blots were col- lected using a PhosphorImager and normalized to the signals for ribosomal phosphoprotein 36B4. For apoli- poprotein <t>C-III</t> (apoC-III), the log fold change was used because this gene is downregulated and its ex- pression ratios lie between 1 and 0. B: Northern blots showing the expression of peroxisomal enoyl-CoA:hy- drotase-3-hydroxyacyl-CoA bifunctional enzyme (bi- functional enzyme), apoC-III, and ribosomal phos- phoprotein 36B4.
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    Image Search Results


    Fig. 4. Relationship between regulated target genes and the relative drug peroxisome proliferator-acti- vated receptor- (PPAR-) efficacy. A: Comparison of quantitative expression levels as measured in microar- rays and Northern blot analysis and the relative PPAR- efficacies of the tested compounds using linear regres- sion analysis. Fold regulations as calculated in Table 2 were used for the microarray data, whereas signal in- tensities for the genes in Northern blots were col- lected using a PhosphorImager and normalized to the signals for ribosomal phosphoprotein 36B4. For apoli- poprotein C-III (apoC-III), the log fold change was used because this gene is downregulated and its ex- pression ratios lie between 1 and 0. B: Northern blots showing the expression of peroxisomal enoyl-CoA:hy- drotase-3-hydroxyacyl-CoA bifunctional enzyme (bi- functional enzyme), apoC-III, and ribosomal phos- phoprotein 36B4.

    Journal: The Journal of Lipid Research

    Article Title: Prediction of PPAR- ligand-mediated physiological changes using gene expression profiles

    doi: 10.1194/jlr.d300239-jlr200

    Figure Lengend Snippet: Fig. 4. Relationship between regulated target genes and the relative drug peroxisome proliferator-acti- vated receptor- (PPAR-) efficacy. A: Comparison of quantitative expression levels as measured in microar- rays and Northern blot analysis and the relative PPAR- efficacies of the tested compounds using linear regres- sion analysis. Fold regulations as calculated in Table 2 were used for the microarray data, whereas signal in- tensities for the genes in Northern blots were col- lected using a PhosphorImager and normalized to the signals for ribosomal phosphoprotein 36B4. For apoli- poprotein C-III (apoC-III), the log fold change was used because this gene is downregulated and its ex- pression ratios lie between 1 and 0. B: Northern blots showing the expression of peroxisomal enoyl-CoA:hy- drotase-3-hydroxyacyl-CoA bifunctional enzyme (bi- functional enzyme), apoC-III, and ribosomal phos- phoprotein 36B4.

    Article Snippet: The purified PCR products were mixed 1:1 in DMSO (Sigma, St. Louis, MO) and rearranged in 384-well plates. cDNA targets were spotted in duplicate onto silanized glass slides (Amersham Biosciences, Buckinghamshire, UK) at 55% relative humidity using the Molecular Dynamics Gen. III microarray spotter.

    Techniques: Comparison, Expressing, Northern Blot, Microarray, Functional Assay